CYTOCOX1

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

• Sinónimos: Cytochrome c Oxidase Activity Assay Kit
• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• Nivel de calidad: 200
• uso: sufficient for 100 tests
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... COX1(4512)

Descripción

Aplicación

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Acciones bioquímicas o fisiológicas

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Características y beneficios

• Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution

Los componentes del kit también están disponibles por separado

• C2506 Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg
• D4641 n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H315,H318
• Consejos de prudencia: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Clasificaciones de peligro: Eye Dam. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 10 - Combustible liquids