C2C12 Cell Line from mouse

91031101, mouse muscle, Myoblast

• Sinónimos: C2-C12 Cells
• UNSPSC Code: 41106514

Propiedades

• product name: C2C12 Cell Line from mouse, C3H muscle myoblast, 91031101
• biological source: mouse muscle
• growth mode: Adherent
• karyotype: Not specified
• morphology: Myoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C3H muscle myoblast

Cell Line Description

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Application

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Culture Medium

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Other Notes

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