Derived from normal subcutaneous connective tissue of an adult C3H strain mouse. The cells were treated with 20 methylcholanthrene and produced sarcomas when injected into C3H strain mice.
Split semi-confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells /cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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