A431 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85090402, human skin, Epithelial

• Sinónimos: A-431 Cells, A431/P Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human skin
• envase: tube of 5 μg 85090402-DNA-5UG; pkg of vial of cells 85090402-1VL
• modo de crecimiento: Adherent
• cariotipo: Hypertriploid
• morfología: Epithelial
• productos: Not specified
• receptores: Epidermal growth factor (EGF)
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human squamous carcinoma

Descripción línea celular

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Aplicación

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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