TR33B

85040102, rat brain, Glial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: TR33B, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85040102
• origen biológico: rat brain
• envase: tube of 5 μg 85040102-DNA-5UG; pkg of vial of cells 85040102-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Glial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat Wistar-Furth oligodendroglioma

Descripción línea celular

Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (Sigma Catalogue number. 85081901).

Aplicación

Tumourigenicity studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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