For testing purposes, this product is prepared in cold deionized water. While PBS has not been tested as a solvent, the enzyme is expected to be soluble. Please see the link below to review the assay protocol for cellulase and hemicellulase:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cellulase
C1794
Cellulase from Trichoderma sp.
Cellulase from Trichoderma sp.
BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Sinónimos:
β-1,4-glucanase
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About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
biological source
fungus (Trichoderma sp.)
Quality Level
product line
BioReagent
form
powder
specific activity
3-10 units/mg solid
contains
maltodextrin
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | plant: suitable
application(s)
agriculture
greener alternative category
, Enabling
storage temp.
2-8°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.
Unit Definition
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
substrate
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Jin Zhou et al.
Bioresource technology, 100(2), 819-825 (2008-09-06)
To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
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Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant
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Protocolos
To standardize an enzymatic assay procedure of cellulase.
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Hi, I was wondering how to prepare the enzyme solution? Can I just dilute it on PBS? Thanks
1 answer-
Helpful?
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Hello, what is the Uniprot number or Gene ID for C1794-5KU? Thank you
1 answer-
The Gene ID for Cellulase from Trichoderma sp. (AKA Gliocladium sp.) is GH45. Please see information at the links below:
Helpful?
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