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C0775

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Crystal Violet
1 of 1 reviewers received a sample product or took part in a promotion

Sinónimos:

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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Acerca de este artículo

Fórmula lineal:
C25H30N3Cl
Número CAS:
548-62-9
Peso molecular:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57653980
EC Number:
208-953-6
MDL number:
MFCD00011750
Beilstein/REAXYS Number:
3580948
Colour Index Number:
42555
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Nombre del producto

Crystal Violet, certified by the BSC

agency

certified by the BSC

Quality Segment

200

form

powder

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

2.5 — 3.5 (20 °C)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes, enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Application

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability.[1] It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

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Este artículo
C615805-671046364
Crystal Violet certified by the BSC

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Crystal Violet

Crystal Violet JIS special grade

Sigma-Aldrich

05-6710

Crystal Violet

Crystal Violet VETRANAL®, analytical standard

Supelco

46364

Crystal Violet

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

color

green to very dark green

color

green to very dark green

color

-

color

-

solubility

H2O: 1 mg/mL

solubility

water: soluble 50 g/L at 27 °C

solubility

-

solubility

water: soluble 50 g/L at 27 °C

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

form

powder

form

powder

form

-

form

-

composition

Dye content, ≥90%

composition

-

composition

-

composition

-

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–3 of 3 Questions  

  1. Can crystal violet or Giemsa be used to stain cells in a saliva/PBS suspension for the purpose of counting neutrophils/mL? What would be the best staining method for this purpose?

    1 answer

    1. Acridine orange is theoretically intended to stain viable cells, and if low intensity is observed, particularly when using a light microscope, it is likely true. However, for visualizing viable cells stained with acridine orange, a fluorescent microscope is routinely used. For simple neutrophil counting in saliva, acridine orange may not be the best choice due to cost considerations.

      While Giemsa solutions can be used, staining can be challenging, and often there are no published procedures for using Wright, Wright Giemsa, or Giemsa solution for counting neutrophils under a hemacytometer. The recommended product for this purpose is Crystal Violet or Turks/Tuerks solution, a simple solution of crystal violet dissolved in water. Acetic acid is sometimes added to the Turks solution to lyse red blood cells, but if the saliva is not bloody, adding acetic acid is unnecessary.

      Helpful?

  2. What is the allowed limit of Crystal Violet before it is considered toxic, carcinogenic, or hazardous?

    1 answer

    1. The most serious hazard listed in the Safety Data Sheet (SDS) is Serious Eye Damage, along with short-term and long-term aquatic hazards. There is also a Category 2 hazard as a carcinogen. However, the risk can be reduced by purchasing prepared solutions instead of preparing them from powder to minimize the risk of inhaling the powder. There are no known safe exposure limits for Crystal Violet. The use of appropriate engineering controls, personal protective equipment, and body protection is recommended.

      Helpful?

  3. Can this be dissolved in ethanol or methanol 

    1 answer

    1. While Crystal Violet solutions used for staining often include ethanol or methanol, this product has not been tested for the direct solubility in either alcohol. This compound is readily soluble in water.

      Helpful?

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1 of 1 reviewers received a sample product or took part in a promotion

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