Once diluted the solution should be stable indefinitely. It should be stored in the refrigerator and shaken well before each use. The solution tends to precipitate on standing and heat accelerates precipitation. Therefore, do not leave the bottle at room temperature overnight.
B2025
Brilliant Blue G - Colloidal Concentrate
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NACRES:
NA.85
UNSPSC Code:
12352200
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Permítanos ayudarleGeneral description
Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.
Application
Brilliant Blue G - Colloidal Concentrate has been used in the staining
- protein fragments post in vitro pepsin digestion
- ovarian tumor fluid proteome prior to profiling
- the transcription factor (TFIID) complex from Saccharomyces cerevisiae
Preparation Note
The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.
Analysis Note
This product has been tested for suitability on SDS-PAGE.
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Este artículo | |||
|---|---|---|---|
| form solution | form liquid | form powder | form methanol solution |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| storage temp. room temp | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Clase de almacenamiento
12 - Non Combustible Liquids
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What is the solution stability of Product B2025, Brilliant Blue G?
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How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
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One bottle of the product makes 1 liter of working solution. The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
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How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
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The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
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Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
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Use with nitrocellulose is not recommended, due to the methanol.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the detection limit of Product B2025, Brilliant Blue G?
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The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
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