As per the Product Information Sheet, the Accutase solution does not contain mammalian or bacterial-derived products. This solution is supplied as a sterile, ready-to use, frozen liquid. This product is serum free.
A6964
Accutase® solution
sterile-filtered, suitable for cell culture
Sinónimos:
Cell detachment solution
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
Concentration:
400-600 units/mL
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlesterility
sterile-filtered
Quality Level
form
liquid
concentration
400-600 units/mL
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
≤1 EU/mL endotoxin
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
General description
La disolución Accutase® (en disolución salina tamponada con fosfato de Dulbecco (DPBS) sin Ca++ ni Mg++) contiene enzimas proteolíticas y colagenolíticas para la separación celular. Es útil para el desprendimiento sistemático de las células del material de plástico para cultivos de tejido convencionales y del material de plástico recubierto por adhesión. Se prefiere Accutase con respecto a la tripsina para el desprendimiento celular, ya que ayuda a separar las células de los recipientes de cultivo con menos daño. Por consiguiente, Accutase suele utilizarse para el tratamiento de células sensibles o para el análisis de marcadores de superficie.
Application
Se ha demostrado eficaz en la separación de los fibroblastos primarios, las células endoteliales, las neuronas, las líneas celulares tumorales y las células de insecto. Su rendimiento es excepcionalmente bueno en la separación de células para análisis de los marcadores de la superficie celular, el análisis del crecimiento vírico y la citometría de flujo, así como en el escalamiento del biorreactor. No contiene productos derivados de mamíferos ni bacterianos.
La disolución Accutase® se ha utilizado para la disociación o la digestión en células individuales de células no adherentes que crecen en agregados, como las células tumorales de cerebro, las células madre pluripotentes (IPSC) y las células de glioblastomas[1].
Adecuada para utilizar en la preparación de suspensiones monocelulares para secuenciación.
Adecuada para utilizar en la preparación de suspensiones monocelulares para secuenciación.
Features and Benefits
Líquido listo para usar compuesto por una mezcla patentada de enzimas proteolíticas y colagenolíticas para utilizar en la disociación de las células del material de plástico convencional o recubierto por adhesión.
Preparation Note
Este producto contiene rojo de fenol. El producto se transporta en hielo seco y podría haber una acumulación significativa de CO2 en el envase. Este CO2 puede entrar en la disolución y bajar ligeramente el pH, lo que provocaría un color amarillo-naranja (alrededor de pH 6,8). La disolución debería poder seguir utilizándose todavía tal cual, o puede agregarse hidróxido de sodio para ajustar el pH. Una botella completamente congelada de Accutase será de color amarillo y virará a naranja a medida que se vaya descongelando. Una vez desempaquetado, el producto puede volver a congelarse o colocarse directamente en el refrigerador para su uso posterior. No descongele el producto en un baño de agua tibia. Accutase es sensible a temperaturas superiores a 37 °C y se inactivará después de 45 minutos a 37 °C. Se recomienda descongelar durante la noche en el refrigerador o en una bañera de agua fría del grifo durante 1-1,5 horas. Agite la botella de Accutase después de descongelar para permitir la disolución uniforme de los ingredientes. Estos productos no necesitan ser calentados antes de ser utilizados. Utilice inmediatamente una vez retirado del refrigerador y vuelva a meterlo en el refrigerador después de su uso.
Preparado en PBS de Dulbecco (0,2 g/l KCl, 0,2 g/l KH2PO4, 8 g/l NaCl y 1,15 g/L Na2HPO4) que contiene EDTA 0,5 mM•4Na y 3 mg/l de rojo de fenol. Consulte la ficha técnica del producto en la página Web para información de uso del producto.
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| concentration 400-600 units/mL | concentration - | concentration - | concentration 1 × |
| form liquid | form liquid | form liquid | form solution |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| sterility sterile-filtered | sterility - | sterility - | sterility sterile-filtered |
| pH 6.8-7.8 | pH - | pH - | pH 6.8-7.8 |
Clase de almacenamiento
12 - Non Combustible Liquids
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Yanting Xue et al.
Stem cell research, 24, 151-154 (2017-10-17)
Human MSX1 gene is mapped to chromosome 4 and encodes a 303aa homeobox protein MSX1. MSX1 expression appears during early tooth development of vertebrate embryogenesis. Mutations in this protein are related to human tooth anomalie, cleft lip and palate and
Autophagy pathways in glioblastoma
Jiang H, et al.
Methods in Enzymology, 453, 273-286 (2009)
Ruchi Bajpai et al.
Molecular reproduction and development, 75(5), 818-827 (2007-12-25)
Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| A6964-100ML | 04061835568079 |
| A6964-500ML | 04061833383094 |
-
Is Accutase Solution A6964 animal-origin free? Is serum free?
1 answer-
Helpful?
-
-
Can accutase be thawed, aliquoted, and refrozen at -20 to preserve it longer?
1 answer-
Yes, this product is stable after undergoing several freeze/thaw cycles. The thawed product may be aliquoted and stored at –20 °C or stored for up to 2 months at 4°C.
The graphic below demonstrates the freeze/thaw stability of Accutase.Helpful?
-
-
On the Innovative cell technologies website it says that accumax is the same as accutase but with 3x the amount of enzymes. Why did the technician here answer that it contains DNAse?
1 answer-
Thank you for calling this to our attention. The manufacturer, Innovative Cell Technologies, has been contacted regarding this matter. The manufacturer has confirmed that both Accutase and Accumax products DO contain a small amounts of DNAse. The concentration is not measured, but it is present in the formulation. In addition, both products also contain 0.5 mM EDTA.
Helpful?
-
-
What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
1 answer-
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
Helpful?
-
-
What is the concentration of Product A6964, Accutase® solution?
1 answer-
This product is typically at a concentration of 400-600 units/mL.
Helpful?
-
-
For what purposes can Product A6964, Accutase® solution, be used?
1 answer-
It can be used for detaching cells, analysis of cell surface markers and virus growth assays.
Helpful?
-
-
How can I inactivate Accutase® with fetal bovine serum?
1 answer-
Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Will Product A6964, Accutase® solution, work on tissue?
1 answer-
This product is not tested for tissue, but it should work on soft tissue.
Helpful?
-
-
What is the difference between Accutase and Accumax?
1 answer-
Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
Helpful?
-
