75259

Tris(2-carboxietil)fosfina hydrochloride

BioUltra, ≥98% (NMR)

• Sinónimos: TCEP
• Fórmula empírica (notación de Hill): C₉H₁₅O₆P · HCl
• Número CAS: 51805-45-9
• Peso molecular: 286.65
• UNSPSC Code: 12352128
• NACRES: NA.25
• PubChem Substance ID: 329766294
• MDL number: MFCD00145469
• Beilstein/REAXYS Number: 3724376

Propiedades

• product line: BioUltra
• Quality Segment: 300
• assay: ≥98% (NMR)
• form: crystals
• reaction suitability: reagent type: reductant
• loss: ≤1.0% loss on drying
• pH: 1.2-1.5
• solubility: H₂O: 1 M, clear, colorless (no residue)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.05 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.05, λ: 280 nm A_max: 0.03
• storage temp.: 2-8°C
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Descripción

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.

Application

Tris(2-carboxyethyl)phosphine hydrochloride has been used:

• as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA
• in reducing sulfhydryl groups to obtain total thiol fraction
• to reduce protein samples

Reactivo hidrosoluble, utilizado para reducción selectiva de disulfuros. Más estable que el DDT y útil en las aplicaciones de espectrometría de masas.

Información de seguridad

• Pictogramas: GHS05
• Palabra de advertencia: Danger
• Códigos de peligro: H314
• Indicaciones de precaución: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• ¿Qué está pasando?: WGK 1