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Documentos clave

247M-9

Sigma-Aldrich

EMA (E29) Mouse Monoclonal Antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

100
500

conjugado

unconjugated

forma del anticuerpo

culture supernatant

tipo de anticuerpo

primary antibodies

clon

E29, monoclonal

descripción

For In Vitro Diagnostic Use in Select Regions (See Chart)

Formulario

buffered aqueous solution

reactividad de especies

human

envase

vial of 0.1 mL concentrate (247M-94)
vial of 0.5 mL concentrate (247M-95)
bottle of 1.0 mL predilute (247M-97)
vial of 1.0 mL concentrate (247M-96)
bottle of 7.0 mL predilute (247M-98)

fabricante / nombre comercial

Cell Marque®

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotipo

IgG2aκ

control

breast

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

visualización

cytoplasmic, membranous

Información sobre el gen

human ... MUC1(4582)

Descripción general

Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. The absence of EMA can also be of value since negative EMA staining is characteristic of some tumors including adrenal carcinoma, seminomas, paraganglioma and hepatoma.

Calidad


IVD
IVD
IVD
RUO

Ligadura / enlace

EMA Positive Control Slides, Product No. 247S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Forma física

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Nota de preparación

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Otras notas

For Technical Service please contact: 800-665-7284 or email: [email protected]

Información legal

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificados de análisis (COA)

Lot/Batch Number
0000430343
0000430343
0000418440
0000418440
0000399656

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D P Dearnaley et al.
British journal of cancer, 44(1), 85-90 (1981-07-01)
We have developed a technique for the immunocytochemical staining of marrow smears using antiserum to epithelial membrane antigen (EMA). This membrane component is confined to, but widely distributed in, epithelial tissues and tumours derived from them, and is strongly expressed
T W Beer et al.
Histopathology, 37(3), 218-223 (2000-09-06)
Seventy-five skin tumours were studied to investigate the value of immunohistochemistry in differentiating basal cell, squamous cell and basosquamous carcinomas of the skin. Archived paraffin-embedded tissue samples of basal cell carcinomas (n = 39), squamous cell carcinomas (n = 23)
M Fraga et al.
American journal of clinical pathology, 103(1), 82-89 (1995-01-01)
Bone marrow involvement by anaplastic large cell anaplastic large cell (ALC) lymphoma can be difficult to detect on routine morphologic examination alone. In a series of 42 patients with ALC lymphoma, the authors analyzed: (1) the usefulness of a limited
G S Pinkus et al.
American journal of clinical pathology, 85(3), 269-277 (1986-03-01)
Epithelial membrane antigen and keratin proteins represent markers of epithelial differentiation that may be detected in routine formalin-fixed, paraffin-embedded tissues. Eighty-seven neoplasms, including 48 adenocarcinomas of various types, squamous and transitional cell carcinomas, small-cell anaplastic carcinomas, carcinoid tumors, mesotheliomas, hepatomas
R L Attanoos et al.
Histopathology, 43(3), 231-238 (2003-08-28)
To evaluate the expression of the intermediate filament desmin in reactive mesothelium and malignant mesothelioma and to compare its utility with five other previously reported immunomarkers claimed to be of use in distinguishing reactive from neoplastic mesothelium. Sixty cases of
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