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Merck

W1761

Millipore

Wilkins Chalgren anaerobic agar

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

cleaning products
clinical testing
cosmetics
food and beverages
personal care

microbiology

suitability

anaerobic bacteria
selective for Bacteroides spp.
selective for Bifidobacterium spp.
selective for Clostridium spp.

Application

Used for the isolation of anaerobic bacteria.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 5.00
Dextrose, 1.00
Sodium chloride, 5.00
L-Arginine, 1.00
Sodium pyruvate, 1.00
Hemin, 0.005
Menadione, 0.0005
Agar, 10.00

Preparation Note

Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates.For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement (Cat. No. N8781) or 2 vial of G.N. Spore Anaerobic Supplement (Cat. No. G3789) as desired into the sterile molten medium before pouring into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Los clientes también vieron

T D Wilkins et al.
Antimicrobial agents and chemotherapy, 10(6), 926-928 (1976-12-01)
A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%)

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