Using P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde was attempted, but the results were not satisfactory with regular formaldehyde. A specific protocol for the reconstitution of Phalloidin P5282-.1MG in the case of tissues fixed in paraformaldehyde and included in O.C.T medium is not available. However, it is suggested to find relevant information in the literature, such as in this article, https://www.oncotarget.com/article/25259/ DOI:[10.18632/oncotarget.25259].
P5282
Phalloidin Peptide
≥90% (HPLC), solid, FITC labeled
Sinónimos:
Phalloidin-FITC
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Fórmula empírica (notación de Hill):
C56H60N10O15S2
Peso molecular:
1177.26
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
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Servicio técnico
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Permítanos ayudarleNombre del producto
Phalloidin, Fluorescein Isothiocyanate Labeled, sequence Amanita phalloides(synthetic: peptide sequence)
biological source
sequence from Amanita phalloides (synthetic: peptide sequence)
Quality Segment
form
solid
technique(s)
protein detection: suitable
fluorescence
λex 495 nm; λem 520 nm(lit.)
storage temp.
−20°C
General description
Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which fluorescently labelled phalloidin compounds can be produced.
Application
Phalloidin, Fluorescein Isothiocyanate Labeled has been used:
- To visualize F-Actin reorganization in primary neonatal cardiomyocytes (PNCMs) and H9C2 cells (rat cardiac myoblasts) following endothelin-1 (ET-1) and angiotensin II (Ang II) treatment.
- In immunochemistry to label microfilament.
- In immunofluorescence analysis to stain F-actin.
Biochem/physiol Actions
Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering. The affinity of phalloidin to actin is not significantly altered after derivatizing fluorescently labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.
Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.
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Este artículo | |||
|---|---|---|---|
| description sequence Amanita phalloides(synthetic: peptide sequence) | description sequence from Amanita phalloides(synthetic: peptide sequence) | description ≥90% | description suitable for fluorescence, ≥80.0% (HPLC) |
| biological source sequence from Amanita phalloides (synthetic: peptide sequence) | biological source sequence from Amanita phalloides (synthetic: peptide sequence) | biological source Amanita phalloides | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form solid | form solid | form powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
| fluorescence λex 495 nm; λem 520 nm(lit.) | fluorescence λex 540-545 nm; λem 570-573 nm | fluorescence - | fluorescence - |
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
1 answer-
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How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
A typical application for staining cells can be found in the product information sheet (under Documents, above).
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How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
The product should be stored at a powder in the freezer at -20°C.
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What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.
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How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.
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