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Merck

11051482001

Roche

dTTP

Lithium salt

Sinónimos:

Thymidine 5′-triphosphate sodium salt, 2′-deoxy-thymidine-5′-triphosphate, deoxy-thymidine-5′-triphosphate, 2′-, TTP, dTTP

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About This Item

Fórmula empírica (notación de Hill):
C10H16N2O14P3Na
Número de CAS:
Peso molecular:
504.15
Número MDL:
Código UNSPSC:
41116100
ID de la sustancia en PubChem:

Nivel de calidad

Ensayo

≥98%

Formulario

solution

uso

sufficient for 6,250 standard reactions (PCR)

envase

pkg of 250 μL (25 μmol)

fabricante / nombre comercial

Roche

concentración

100 mM

técnicas

DNA amplification: suitable
DNA sequencing: suitable

temp. de almacenamiento

−20°C

cadena SMILES

[NaH].[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)C)O)(O[P](=O)(O)O)O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

Clave InChI

KAELIXTVSANDSE-UHFFFAOYSA-N

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Descripción general

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

Aplicación

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

Calidad

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Definición de unidad

1 μmol dTTP = 9.5 A260units

Otras notas

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

No data available

Punto de inflamabilidad (°C)

No data available


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Memorias do Instituto Oswaldo Cruz, 103(5), 455-462 (2008-09-18)
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture
A Pillay et al.
Journal of clinical microbiology, 42(8), 3853-3856 (2004-08-07)
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet
Wieland Meyer et al.
Emerging infectious diseases, 9(2), 189-195 (2003-02-27)
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase

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