The recommended volume per gel is 5ul. The package volume is 500ul, thus it is sufficient for 100 lanes or gels. The product is packaged in a 25ml bottle.
69079
Perfect Protein Markers, 10-225 kDa
Sinónimos:
protein markers, protein molecular weight markers, protein standards
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
41105337
NACRES:
NC.01
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Formulario
liquid
Nivel de calidad
fabricante / nombre comercial
Novagen®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
Características y beneficios
• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
• Recombinant, unmodified markers producesharp, accurately sized bands
• Available in two size ranges:
15-150 kDa
10-225 kDa
Advertencia
Toxicity: Toxic (F)
Información legal
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Csenge Filep et al.
Analytical chemistry, 92(5), 4023-4028 (2020-02-06)
The temperature-dependent migration of molecular weight protein size standards and several biotherapeutic proteins were studied in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) in the interval from 15 to 60 °C using borate cross-linked dextran sieving matrix. Arrhenius plots were
Rebeccah A Warmack et al.
The Journal of biological chemistry, 294(32), 12203-12219 (2019-06-27)
Transparency in the lens is accomplished by the dense packing and short-range order interactions of the crystallin proteins in fiber cells lacking organelles. These features are accompanied by a lack of protein turnover, leaving lens proteins susceptible to a number
-
Hi, Can you please let know the packaging volume and the container that the product will be supplied? The packaging details is a bit confusing, as it states 25ml and 100 lane. And also please provide the details of how many microliters to be added to gel?
1 answer-
Helpful?
-
-
Hello, Please; I used your product Perfect Protein™ Markers, 10-225 kDa, in my Lab for SDS. However, I do not know the band range interpretation. Please, I need assistance with the band range interpretation. I will appreciate any document showing such.
1 answer-
As mentioned on the product detail page, under the "Description" section, the Perfect Protein Markers, 10-225 kDa, include the protein sizes of 10, 15, 25, 35, 50, 75,100,150, and 225kDa . Please refer to page 72 of the brochure linked on the product detail page to see the image of the 10-225 kDa markers.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
