573115

SMCC, Water-Soluble

• Sinónimos: SMCC, Water-Soluble, Sulfosuccinimidyl- trans-4-(N-maleimidomethyl)cyclohexane-1-carboxylate, Sulfo-SMCC, Sulfosuccinimidyl-trans-4-(N-maleimidomethyl)cyclohexane-1-carboxylate, Sulfo-SMCC
• Fórmula empírica (notación de Hill): C₁₆H₁₇N₂O₉S · Na
• Número de CAS: 92921-24-9
• Peso molecular: 436.37
• Número MDL: MFCD00054978
• Código UNSPSC: 41116133
• NACRES: NA.23

Propiedades

• Ensayo: ≥94% (HPLC)
• Nivel de calidad: 100
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: off-white
• solubilidad: water: 2-5 mg/mL
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [Na+].[S](=O)(=O)([O-])C1CC(=O)N(C1=O)OC(=O)C2CCC(CC2)CN3C(=O)C=CC3=O
• InChI: 1S/C16H18N2O9S.Na/c19-12-5-6-13(20)17(12)8-9-1-3-10(4-2-9)16(23)27-18-14(21)7-11(15(18)22)28(24,25)26;/h5-6,9-11H,1-4,7-8H2,(H,24,25,26);/q;+1/p-1
• Clave InChI: VUFNRPJNRFOTGK-UHFFFAOYSA-M

Descripción

Descripción general

A water-soluble derivative of SMCC. Ideal for coupling enzymes to antibodies. Contains a cyclohexane bridge, which gives extra stability to the maleimide group. Both enzyme activity and antibody specificity are preserved after coupling. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.

Ideal for coupling enzymes to antibodies. Contains a cyclohexane bridge, which gives extra stability to the maleimide group. Both enzyme activity and antibody specificity are preserved after coupling.

Advertencia

Toxicity: Irritant (B)

Reconstitución

Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid SMCC can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.

Otras notas

D’Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable