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V1514

Sigma-Aldrich

Anti-VE-Cadherin (CD144) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-CD144, Anti-Cadherin-5, Anti-Vascular Endothelial Cadherin

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~130 kDa

Espèces réactives

human

Technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil
western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CDH5(1003)

Description générale

Cadherin 5 (CDH5), also known as cadherin 144 (CD144), is encoded by the gene mapped to long arm of human chromosome 16. The encoded protein is expressed in endothelial cells, but it absent in normal epithelium.
VE-Cadherin (also designated vascular endothelial cadherin, cadherin-5, and cluster of differentiation 144 (CD144)) is a non-type I or II cadherin that is expressed on endothelial cells of all blood and lymphatic vessels, where it organizes intercellular adherens junctions.

Immunogène

synthetic peptide corresponding to amino acids 91-110 of human VE-cadherin (CD144).

Application

Anti-VE-Cadherin (CD144) antibody produced in rabbit has been in following studies:
  • immunofluorescence imaging
  • western blotting
  • to weaken cell-cell junctions in human umbilical vein endothelial cells (HUVECs)
  • immunohistochemistry

Actions biochimiques/physiologiques

Cadherin 5 (CDH5)/CD144 plays an essential role in various cellular processes, such as regulation of cell adhesion, cell proliferation, cell survival, cell shape, cell motility. Additionally, it is also involved in the regulation of signaling pathways and transcriptional gene regulation. CD144/VE-cadherin is a core component of endothelial adherens junction, which plays a vital role in angiogenic sprouting by enhancing cell migration and cell elongation. CD144 expressed in glioblastoma multiforme (GBM) facilitates the transdifferentiation of glioblastoma stem like cells (GSCs) to endothelial cells and form blood vessels, under hypoxia condition. VE-cadherin induces endothelial cell-cell adhesion at adherens junctions (AJs). CD144 gene knockout results in loss of cell polarity and modification in vascular lumenal structure. CD144 inhibits the synthesis of CDH2 (also known as N-cadherin) in the endothelium.
VE-cadherin (CD144) interacts with β-catenin, plakoglobinand Adherens junction protein (p120ctn).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A lung-on-a-chip array with an integrated bio-inspired respiration mechanism.
Stucki AO
Lab on a chip, 15, 1302-1310 (2015)
Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity.
Kremmidiotis G
Genomics, 49, 467-471 (1998)
Effects of Morphology vs. Cell?Cell Interactions on Endothelial Cell Stiffness
Stroka KM and Aranda-Espinoza H.
Cellular and Molecular Bioengineering, 4, 9-27 (2011)
Manon C A Pronk et al.
Small GTPases, 10(6), 466-484 (2017-09-28)
RhoGTPases are known regulators of intracellular actin dynamics that are important for maintaining endothelial barrier function. RhoA is most extensively studied as a key regulator of endothelial barrier function, however the function of the 2 highly homologous family-members (> 88%)
Functionally specialized junctions between endothelial cells of lymphatic vessels
Baluk P, et al.
The Journal of Experimental Medicine, 204(10), 2349-2349 (2007)

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