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SRP8026

Sigma-Aldrich

Annexin V human

recombinant, expressed in E. coli, GST tagged, ≥95% (SDS-PAGE)

Synonyme(s) :

Annexin A5, Calphobindin I, EC 2.7.1.23, Endonexin II, Placental anticoagulantprotein I

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Étiquette/Marqueur

GST tagged

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

~52 kDa

Conditionnement

pkg of 100 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

activity assay: suitable

Couleur

white

Numéro d'accès UniProt

P08758

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... ANXA5(308)

Description générale

Annexin V is a member of the annexin family of calcium and membrane-binding proteins that have anticoagulatory and anti-inflammatory properties. The encoded protein is 320 amino acid long and is folded into four domains, each having five α-helices, forming a superhelical globular structure. The ANX5 gene is mapped to human chromosome 4q26-q28 and spans a length of 28kb containing 13 exons and 12 introns.

Application

Annexin V has been used to examine phosphorylation activity of ChaK1 (channel-kinase 1).

Actions biochimiques/physiologiques

Annexins are a family of water-soluble proteins that bind to membranes and phospholipids in a calcium-dependent manner. They participate in several cellular functions, such as membrane fusion and cytoskeletal interaction. Annexin V is a potent anticoagulant and serves as a voltage-gated calcium channel when bound to membrane in vitro. It is found to inhibit phospholipase A2 and protein kinase C.

Forme physique

Lyophilized from PBS, pH 7.4.

Reconstitution

Reconstitute with 100 μL deionized water.

Autres remarques

Recombinant human annexin V fused to a GST-tag.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Claire Pluchart et al.
Journal of thrombosis and thrombolysis, 48(2), 195-202 (2019-06-09)
Thromboembolic events are frequent and serious complications of acute lymphoblastic leukaemia treatment. The importance of chemotherapy in the pathogenesis of this increased risk is enhanced by the fact that thrombosis rarely occurs at diagnosis. Our study aims at investigating the
Phosphorylation of annexin I by TRPM7 channel-kinase.
Dorovkov MV
The Journal of Biological Chemistry, 279, 50643-50646 (2004)
Megan L Sulciner et al.
The Journal of experimental medicine, 215(1), 115-140 (2017-12-02)
Cancer therapy reduces tumor burden by killing tumor cells, yet it simultaneously creates tumor cell debris that may stimulate inflammation and tumor growth. Thus, conventional cancer therapy is inherently a double-edged sword. In this study, we show that tumor cells
Thomas Exner et al.
Clinical chemistry and laboratory medicine, 57(5), 690-696 (2018-11-15)
Background The aim of the study was to investigate the specificity of an activated charcoal-based product (DOAC Stop™) initially intended for the specific extraction of direct oral anticoagulants (DOACs) from test plasmas on a range of other anticoagulants. Methods Test
Marielle E Yohe et al.
Science translational medicine, 10(448) (2018-07-06)
The RAS isoforms are frequently mutated in many types of human cancers, including PAX3/PAX7 fusion-negative rhabdomyosarcoma. Pediatric RMS arises from skeletal muscle progenitor cells that have failed to differentiate normally. The role of mutant RAS in this differentiation blockade is
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