SRP3018
Beta -NGF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
beta-Nerve Growth Factor
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in E. coli
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Puissance
<1.0 ng/mL ED50
Poids mol.
13.5 kDa
Conditionnement
pkg of 20 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... NFG(4803)
Description générale
NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It was discovered by Levi-Montalcini, and is the founding member of the neurotrophin and belongs to the NGF family.
Application
NGFβ (nerve growth factor β) has been used for the treatment of normal human bronchial epithelial cells (NHBE), to determine its influence on ion transport in the presence of amiloride, NPPB, and ouabain. It has also been used for the apical incubation with MKHS (modified Krebs-Henseleit solution) in cells to determine its stability and concentration post- prolonged incubation periods in MKHS.
Actions biochimiques/physiologiques
NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGFβ signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.
Séquence
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR
Forme physique
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a Stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Over-expression of nerve growth factor-? in human cholangiocarcinoma QBC939 cells promote tumor progression.
Yue XJ, et al.
PLoS ONE, 8(4) (2013)
The nerve growth factor 35 years later.
R Levi-Montalcini
Science (New York, N.Y.), 237(4819), 1154-1162 (1987-09-04)
Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells.
Shimko MJ, et al.
Physiological Reports, 2(7) (2014)
Li Lin et al.
Molecules (Basel, Switzerland), 24(10) (2019-05-28)
Two optical isomers, +/- gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (1H-1H COSY, HMBC, HSQC) NMR spectroscopy and
Urinary nerve growth factor could be a biomarker for interstitial cystitis/painful bladder syndrome: a meta-analysis.
Qu HC, et al.
PLoS ONE, 9(9) (2014)
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