SAE0117
Amelogenin, human
X isoform, recombinant, expressed in E. coli
Synonyme(s) :
AIH1, AMELX, AMG, AMGX, Amel
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26
Produits recommandés
Source biologique
human
Produit recombinant
expressed in E. coli
Pureté
≥90% (HPLC)
Forme
solid
Impuretés
≤1 EU/μg
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... AMELX(265)
Description générale
Research area: Neuroscience
Amelogenin, X isoform belongs to the family of closely related amelogenin proteins that participate in the amelogenesis process of teeth mineralization. Amelogenins account for more than 90% of the total protein in developing tooth enamel.
Amelogenin, X isoform belongs to the family of closely related amelogenin proteins that participate in the amelogenesis process of teeth mineralization. Amelogenins account for more than 90% of the total protein in developing tooth enamel.
Actions biochimiques/physiologiques
Mutations in AMELX are responsible for the development of the rare disease Amelogenesis imperfecta 1E (AI1E), which is characterized by abnormal tooth enamel development. Amelogenins act both as attachment factors and as a growth factor for mesenchymal stem cells. Amelogenin is of increasing importance as a potential therapeutic agent for variety of application including treatment hard-to-heal wounds, promoting remineralization of caries lesions, and repairing human tooth enamel.
Notes préparatoires
The product can be reconstituted in water or 2% acetic acid in a concentration of 0.1-5 mg/ml. Tap vial to make sure protein is reconstituted. Aliquot reconstituted Amelogenin and store at -20 °C. Avoid repeated freeze/thaw cycles. Amelogenin may form thin clear film on some surfaces, leading to reduced concentration in solution. Exact Amelogenin concentration can be determined by absorption at 280nm. Amelogenin solution of 0.1% has A280=1.35
Autres remarques
The product is supplied as lyophilized powder, prepared from a 0.2 μM filtered solution of Amelogenin in 2% acetic acid without any additives. The lyophilized product is essentially salt free. The biological activity of recombinant, human AMELX is measured by its ability to promote attachment of Saos-2 cells to a coated surface. The ED50 is defined as the amount of AMELX per cm2 that elicits 50% of the maximal attachment activity.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Regeneration of bone and periodontal ligament induced by recombinant amelogenin after periodontitis.
Haze, A. et al.
Journal of Cellular and Molecular Medicine, 13(6), 1110-1124 (2009)
M Lagerström-Fermér et al.
Genomics, 26(1), 159-162 (1995-03-01)
Formation of tooth enamel is a poorly understood biological process. In this study we describe a 9-bp deletion in exon 2 of the amelogenin gene (AMGX) causing X-linked hypoplastic amelogenesis imperfecta, a disease characterized by defective enamel. The mutation results
Masanobu Izumikawa et al.
TheScientificWorldJournal, 2012, 879731-879731 (2012-05-02)
The aim of this study was to clarify the function of amelogenin, the major protein of enamel matrix derivative, on the proliferation, differentiation, and mineralization of cultured rat bone marrow stem cells (BMSCs), toward the establishment of future bone regenerative
Frank Schwarz et al.
Clinical oral investigations, 8(3), 165-171 (2004-04-03)
The purpose of the present study was to investigate the effects of an enamel matrix protein derivative (EMD) on attachment, proliferation, and viability of human SaOs(2) osteoblasts on titanium implants. A total of 220 sand-blasted and acid-etched (SLA) titanium discs
Marco Romanelli et al.
Clinical interventions in aging, 3(2), 263-272 (2008-08-09)
Amelogenins are extracellular matrix proteins that, under physiological conditions, self-assemble into globular aggregates up to micron-sizes. Studies with periodontal fibroblasts indicate that attachment to these structures increases the endogenous secretion of multiple growth factors and cell proliferation. Pre-clinical and clinical
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