SAB4200646

Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse

clone ALIX-1, purified from hybridoma cell culture

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: ALIX-1, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): flow cytometry: 5-10 μg/test using using human MOLT4 cells; immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.; immunofluorescence: 10-20 μg/mL using using human HeLa cells; immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.
• Isotype: IgG1
• Numéro d'accès UniProt: Q8WUM4
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PDCD6IP(10015)

Description

Description générale

Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).

Immunogène

peptide corresponding to an internal region of human ALIX/ PDCD6IP

Application

Monoclonal Anti-ALIX/PDCD6IP antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

Actions biochimiques/physiologiques

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable