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Key Documents

SAB4200174

Sigma-Aldrich

Anti-Rab18 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-RAB18LI1, Anti-RP11-148B2.1, Anti-ras-related protein Rab-18

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

~23 kDa (additional bands of higher MW may appear in some extract preparations)

Espèces réactives

rat, mouse, human

Conditionnement

antibody small pack of 25 μL

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using lysates of human PC3 cells
indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells
western blot: 2.5-5.0 μg/mL using whole extracts of mouse MM3MG cells

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAB18(22931)
mouse ... Rab18(19330)
rat ... Rab18(307039)

Description générale

Ras-related protein Rab-18 (Rab18) is a member of the ras-associated binding (Rab) family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif, implicated in subcellular targeting. RAB18 gene is mapped to human chromosome 10p12.1.

Spécificité

Anti-Rab18 (C-terminal) recognizes human, mouse, and rat Rab18.

Application

Anti-Rab18 (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Actions biochimiques/physiologiques

Ras-associated binding (Rab)-guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. It localizes to lipid droplets and induces the association of the lipid droplets with endoplasmic reticulum (ER) membranes. Anti-Rab18 may thus be used as a marker to follow the dynamics of lipid droplets in living cells. Rab18 has a potential function in eye development. Mutations in the RAB18 are implicated in Warburg micro syndrome.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Stockage et stabilité

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Discard working dilutions if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Shintaro Ozeki et al.
Journal of cell science, 118(Pt 12), 2601-2611 (2005-05-26)
Lipid droplets (LDs) are organelles that store neutral lipids, but their regulatory mechanism is not well understood. In the present study, we identified Rab18 as an LD component of HepG2 cells by proteomic analysis, and confirmed its localization by immunohistochemistry
Selma Yilmaz Dejgaard et al.
Cellular and molecular life sciences : CMLS, 76(10), 1935-1945 (2019-03-05)
Rab18 is one of the small number of conserved Rab proteins which have been traced to the last eukaryotic common ancestor. It is found in organisms ranging from humans to trypanosomes, and localizes to multiple organelles, including most notably endoplasmic
Olena Pylypenko et al.
Small GTPases, 9(1-2), 22-48 (2017-06-21)
Rab molecular switches are key players in defining membrane identity and regulating intracellular trafficking events in eukaryotic cells. In spite of their global structural similarity, Rab-family members acquired particular features that allow them to perform specific cellular functions. The overall
A case report of a patient with interstitial duplication of 10p12. 1 and 15q11. 2q13. 1
Park HY, et al.
European Journal of Paediatric Neurology, 21, e48-e48 (2017)
Danai Bem et al.
American journal of human genetics, 88(4), 499-507 (2011-04-09)
Warburg Micro syndrome and Martsolf syndrome are heterogenous autosomal-recessive developmental disorders characterized by brain, eye, and endocrine abnormalities. Previously, identification of mutations in RAB3GAP1 and RAB3GAP2 in both these syndromes implicated dysregulation of the RAB3 cycle (which controls calcium-mediated exocytosis

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