RAB0408
Mouse Prolactin ELISA Kit
for serum, plasma and cell culture supernatant
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About This Item
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.84
Produits recommandés
Espèces réactives
mouse
Conditionnement
kit of 96 wells (12 strips x 8 wells)
Technique(s)
ELISA: suitable
capture ELISA: suitable
Entrée
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL
Méthode de détection
colorimetric
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
mouse ... Prl(19109)
Description générale
The Mouse Prolactin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma, cell culture supernatants and urine.
Immunogène
Recombinant Mouse Prolactin
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Actions biochimiques/physiologiques
Prolactin (Prl) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins, which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation and regulation of Bcl-2 protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens. Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils and dendritic cells and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands.
Autres remarques
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)FDS
- RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
- RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)FDS
- RABSTOP3ELISA Stop Solution (Item I)FDS
- RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
- RABWASH420X Wash Buffer (Item B)FDS
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Impaired mammary gland T cell population during early lactation in hypoprolactinemic lactation-deficient rats.
Mackern-Oberti JP
Reproduction (Cambridge, England) null
N-terminal prolactin-derived fragments, vasoinhibins, are proapoptoptic and antiproliferative in the anterior pituitary.
Ferraris J
PLoS ONE null
Conditional Deletion of the Prolactin Receptor to Identify Brain-Specific Actions of Prolactin
3 Biotech null
John D Landua et al.
Journal of mammary gland biology and neoplasia, 25(2), 145-162 (2020-07-25)
Targeted disruption of the murine Hoxd10 gene (ΔHoxd10) leads to a high frequency of localized (gland-to-gland or regionally within a gland) lactation impairment in homozygous mutant mice as a single gene mutation. The effect of Hoxd10 disruption was enhanced by
A N Johnston et al.
Breast cancer research : BCR, 20(1), 42-42 (2018-05-21)
Psychiatric medications are widely prescribed in the USA. Many antipsychotics cause serum hyperprolactinemia as an adverse side effect; prolactin-Janus kinase 2 (JAK2)-signal transducer and activator of transcription 5 (STAT5) signaling both induces cell differentiation and suppresses apoptosis. It is controversial
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