This medium is recommended to be sterilized by 0.22 micron filtration. Autoclaving has not been evaluated for this product.
R6504
Milieu RPMI-1640
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
Roswell Park Memorial Institute 1640 medium
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form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
Le milieu RPMI 1640 a été mis au point par Moore et ses collaborateurs, au Roswell Park Memorial Institute (RPMI), en 1966. Version modifiée du milieu 5A de McCoy, il a été formulé pour permettre la culture de cellules lymphoblastoïdes en suspension, mais on sait depuis qu'il convient aussi à une grande diversité de cellules adhérentes. Initialement conçu pour être utilisé avec un supplément à base de sérum, le milieu RPMI 1640 s'est révélé adapté à la culture sans sérum de différentes lignées cellulaires. Il est aussi couramment employé dans les protocoles de fusion ainsi que pour la croissance de cellules hybrides. Ce milieu convient à la culture de leucocytes normaux et néoplasiques humains.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.
Application
RPMI-1640 Medium is used for the following applications:
- used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)[1]
- used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)[2]
- used for cell isolation and culture[3][4]
- used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay[5]
- used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays[6]
- used for the preparation of antifungal agents[7][8]
Preparation Note
Formulated to contain 10.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.
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Cet article | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components L-glutamine: yes | components L-glutamine: yes | components sodium pyruvate: no | components HEPES: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level - | Quality Level 500 | Quality Level - |
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Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
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Is this medium formulation autoclavable?
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Is the RPMI-1640 powder sterile or is it required to sterilise it after formulation?
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Sterility testing is not performed for this product. Please refer to the Certificate of Analysis for the tested specifications.
https://www.sigmaaldrich.com/product/sigma/r6504#product-documentation
The preparation instructions, including a recommended sterilization method, can be found in the product datasheet.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/388/175/r6504pis.pdfHelpful?
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R6504の粉末培地を購入しました。組成表はどのように入手したら良いでしょうか？
1 answer-
Please see the link below to review the product datasheet which includes the formulation:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/388/175/r6504pis.pdfHelpful?
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how much media is added to prepare 10ml rpmi media?
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This product is a powder form of RPMI. This formulation requires 10.4 grams to prepare one liter of medium. The addition of 2.0 g/L sodium bicarbonate is also required. To prepare 10 ml of liquid medium from this item would require 104 mg of the powder product plus the addition of 20 mg of sodium bicarbonate. Note that R8758, the prepared liquid medium of this version of RPMI, may be of interest.
Please see the link below to compare the powder and liquid medium formulations:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdfHelpful?
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Why doesn't the powder dissolve in deionised water for 3 days?
1 answer-
The product issue you are reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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Buongiorno Gentilissima/o, volevo chiedervi maggiori informazioni per quanto riguarda la differenza tra il terreno R6504-10L e il R4130-10L: sono entrambi in polvere? da cosa deriva la differenza di prezzo? funziona per la crescita di muffe? Grazie
1 answer-
The only difference between these two formulations is the addition of HEPES buffer to the R4103. All other components are identical. None of the classical mammalian culture media are tested for culture or expansion of molds. However, sterile solutions of this media, if handled improperly, have been known to become easily and heavily contaminated the airborne contaminants such as mold spores. To compare these media formulations please see the link below to access the RPMI-1640 formulations table:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/209/459/r6504for.pdfHelpful?
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is this product available in powder form
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Product R6504 is a powdered formulation of RPMI. It is formulated to contain 10.4 grams of powder per liter of medium and requires supplement with 2.0 g/L sodium bicarbonate. Its 1X liquid product equivalent is Product R8758 (https://www.sigmaaldrich.com/US/en/product/sigma/r8758
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