PLX15
GenElute™ Plasmid Maxiprep Kit
sufficient for 15 purifications
Caractéristiques en matière de développement durable
Synonyme(s) :
Gen Elute, GenElute™ Plasmid Kit
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41105501
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderusage
sufficient for 15 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: ~45 min kit run time, sample volume: 25-200 mL bacterial culture
storage temp.
15-25°C
General description
The GenElute Plasmid Maxiprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 1.2 mg of plasmid DNA can be recovered from 25-200 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The recovered plasmid DNA is predominantly in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Central nervous system
Classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Que se passe-t-il ?
WGK 3
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.