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Key Documents

P6649

Millipore

Protein A–Sepharose 6MB

aqueous ethanol suspension

Synonyme(s) :

Protein A–Agarose macrobeads

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About This Item

Numéro MDL:
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

aqueous ethanol suspension

Ampleur du marquage

~1 mg per mL

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino

Espaceur de matrice

1 atom

Capacité

~6 mg/mL binding capacity (human IgG)

Température de stockage

2-8°C

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Description générale

P6649-10ml′s updated product number is GE17-0469-01

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A–Sepharose 6MB has been used in immunoprecipitation.

Forme physique

Suspension in 20% ethanol

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

100.4 °F

Point d'éclair (°C)

38 °C


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Consulter la Bibliothèque de documents

Gaurav Dugar et al.
Molecular cell, 69(5), 893-905 (2018-03-03)
Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter
Nikolai Peschek et al.
The EMBO journal, 38(16), e101650-e101650 (2019-07-18)
Small regulatory RNAs (sRNAs) are crucial components of many stress response systems. The envelope stress response (ESR) of Gram-negative bacteria is a paradigm for sRNA-mediated stress management and involves, among other factors, the alternative sigma factor E (σE ) and
G V Hillyer et al.
The American journal of tropical medicine and hygiene, 38(3), 547-552 (1988-05-01)
Total RNA containing messenger RNA has been isolated from adult Fasciola hepatica and translated in vitro using the rabbit reticulocyte lysate system. L-[35S]-methionine labeled translation products have been immunoprecipitated with sera collected from rabbits infected with F. hepatica, rabbits immunized
Sundar Rajan Selvaraj et al.
Molecular biology of the cell, 19(12), 5579-5592 (2008-09-26)
Retinol-binding protein (RBP) is secreted out of the cell in its ligand-bound holo-form. The apo-form of RBP is selectively retained within the endoplasmic reticulum (ER) by a mechanism that remains unknown. Using isolated microsomal system, we have recapitulated the biogenesis
L F Wang et al.
Gene, 169(1), 53-58 (1996-02-22)
Epitope tagging (Eta) is becoming an increasingly useful technique in molecular biology and biotechnology for the detection, characterisation and purification of recombinant proteins (re-proteins). Here we describe a novel Eta system composed of two different monoclonal antibodies (mAb; D11 and

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