NA9604

GenElute^™ HP 96-Well Plasmid Miniprep Kit

sufficient for 4 96-well plate purifications

• Synonyme(s) : 96 well plasmid extraction kit, 96 well plasmid isolation kit, 96 well plasmid purification kit, High Throughput Purification, Plasmid Purification, high throughput miniprep kit, high throughput plasmid kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute, GenElute^™ HP Plasmid Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Propriétés

• usage: sufficient for 4 96-well plate purifications
• Quality Segment: 200
• technique(s): DNA purification: suitable
• test parameters: : <50 min kit run time, sample volume: 1.3 mL bacterial culture
• storage temp.: 15-25°C

Description

General description

The GenElute HP 96-Well Plasmid Miniprep Kit offers a simple, rapid, cost effective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Click here to view the kit protocol

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.

The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

Application

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Features and Benefits

• Up to 10 μg of high-copy plasmid DNA per well
• Flexibility of both manual and automated formats
• Optimized for production of virus titers from MISSION shRNA plasmid vectors
• No phenol/chloroform extraction or alcohol precipitation required

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

• CLS3879

  Corning^® 96 well clear polypropylene corner notch microplate, round bottom clear, polypropylene, individually wrapped, sterile, lid 4

  FDS
• C2112

  Column Preparation Solution

  FDS
• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  FDS
• L1912

  Lysis Solution

  FDS
• W4639

  Wash Solution 2

  FDS
• R6148

  RNase A solution

  FDS
• W0263

  Wash Solution 1

  FDS

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Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05,GHS07
• Mot-clé: Danger
• Mentions de précaution: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Organes cibles: Central nervous system
• Classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 77.0 °F
• Point d'éclair (°C): 25 °C
• Que se passe-t-il ?: WGK 3
• Codes de danger: H226,H290,H302 + H332,H314,H334,H336
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3