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M7815

Sigma-Aldrich

Anti-MDM2 antibody ,Mouse monoclonal

clone HDM2-323, purified from hybridoma cell culture

Synonyme(s) :

Anti-ACTFS, Anti-HDMX, Anti-LSKB, Anti-hdm2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

HDM2-323, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~90 kDa

Espèces réactives

human, mouse

Concentration

~2 mg/mL

Technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL (whole cell extract of 293T cells transfected with human MDM2)

Isotype

IgG2a

Numéro d'accès UniProt

Q00987

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MDM2(4193)
mouse ... Mdm2(17246)

Description générale

Monoclonal Anti-MDM2 (mouse IgG2a isotype) is derived from the HDM2-323 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. MDM2 (the human homologue of Mdm2, a 90 kDa molecule), is a transcriptional target of p53.

Immunogène

synthetic peptide corresponding to amino acids 389-402 of human MDM2 conjugated to KLH.

Application

Anti-MDM2 antibody, Mouse monoclonal has been used in western blotting and enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

MDM2 functions in the activation of p53 resulting in MDM2 expression, which consequently inhibits p53 transcriptional function. MDM2 accomplishes this inhibition in two ways: because of its physical interaction with p53, MDM2 both represses p53 transcriptional activity and mediates the degradation of p53. Overexpression of MDM2 results in reduced quantities of coexpressed p53, and disruption of the p53-MDM2 interaction by mutation results in both activation and accumulation of p53. MDM2 protein has been shown to activate cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/DP1.5 The amplification of the MDM2 gene has been demonstrated in various human neoplasms.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53
Fang S, et al.
The Journal of Biological Chemistry, 275(12), 8945-8951 (2000)
Dan Zhu et al.
The Journal of clinical investigation, 125(4), 1497-1508 (2015-03-10)
Synaptic plasticity is the ability of synapses to modulate the strength of neuronal connections; however, the molecular factors that regulate this feature are incompletely understood. Here, we demonstrated that mice lacking brain-specific angiogenesis inhibitor 1 (BAI1) have severe deficits in
Xiaobin Wu et al.
OncoTargets and therapy, 11, 3111-3117 (2018-06-07)
The role of dysfunction of MCPH1, a recently identified tumor suppressor gene, has not yet been established in lung cancer. In our previous study, it was reported that MCPH1 expression is downregulated in lung cancer tissues and that MCPH1 overexpression
Antonella Falconieri et al.
Scientific reports, 10(1), 15850-15850 (2020-09-29)
Mutations of the von Hippel-Lindau (pVHL) tumor suppressor are causative of a familiar predisposition to develop different types of cancer. pVHL is mainly known for its role in regulating hypoxia-inducible factor 1 α (HIF-1α) degradation, thus modulating the hypoxia response.
Yu-Hui Li et al.
Frontiers in oncology, 10, 567-567 (2020-05-22)
Global incidence and mortality associated with hepatocellular carcinoma (HCC) is steadily increasing. Metastasis-associated 1 (MTA1) can induce tumorigenesis and metastatic progression in HCC. However, the mechanistic details of MTA1-mediated regulation of HCC has not been completely defined. Epigenetic histone modification

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