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H8509

Sigma-Aldrich

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

Synonyme(s) :

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH

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About This Item

Numéro CAS:
9028-38-0
Numéro de classification (Commission des enzymes):
1.1.1.30 (BRENDA, IUBMB)
Numéro CE :
232-830-6
Numéro MDL:
MFCD00130633
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Type

Type V

Forme

lyophilized powder

Activité spécifique

250-750 units/mg protein

Produit purifié par

chromatography

Activité étrangère

lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%

Température de stockage

−20°C

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Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Actions biochimiques/physiologiques

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Définition de l'unité

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Forme physique

Lyophilized powder containing Tris buffer salts

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Lara Hasan et al.
Therapeutic drug monitoring, 33(6), 757-765 (2011-11-23)
Gamma-hydroxybutyric acid (GHB) has become one of the most dangerous illicit drugs of abuse today. It is used as a recreational and date rape drug because of its depressant effect on the central nervous system, which may cause euphoria, amnesia
J Nie et al.
Scandinavian journal of medicine & science in sports, 21(5), 625-629 (2010-05-13)
This study examined the response of serum biomarkers of cardiac and skeletal muscle damage at rest and after a routine workout of 21 km run in 12 male adolescent (16.2±0.6 years) long-distance runners. Biomarkers of cardiac [troponins (cTnT, cTnI), creatine
Jörn Oliver Sass
Journal of inherited metabolic disease, 35(1), 23-28 (2011-04-12)
Ketone bodies acetoacetate and 3-hydroxy-n-butyric acid are metabolites derived from fatty acids and ketogenic amino acids such as leucine. They are mainly produced in the liver via reactions catalyzed by the ketogenic enzymes mitochondrial 3-hydroxy-3-methylglutary-coenzyme A synthase and 3-hydroxy-3-methylglutary-coenzyme A
Yanfeng Zhang et al.
Acta biochimica et biophysica Sinica, 43(12), 996-1002 (2011-11-01)
Succinic semialdehyde reductase (SSAR) is an important enzyme involved in γ-aminobutyrate (GABA) metabolism. By converting succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB), the SSAR facilitates an alternative pathway for GABA degradation. In this study, we identified SSARs from Geobacter sulfurreducens and
Michel A Sciotti et al.
Chimia, 64(11), 793-798 (2011-01-05)
Gamma hydroxybutyric acid (GHB) is a regulated therapeutic drug, which naturally occurs in mammalian brain tissues as an intermediate of the GABA (gamma aminobutyric acid) neurotransmitter metabolism. The increasing misuse of GHB as a narcotic or abusing drug in recent
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