GE28-4009-89
His MultiTrap™ High Performance
Cytiva 28-4009-89, pack of 4 plates
Synonyme(s) :
High Performance His Trap, His MultiTrap, MultiTrap for His
About This Item
Produits recommandés
Matériaux
polypropylene and polyethylene
Conditionnement
pack of 4 plates
Fabricant/nom de marque
Cytiva 28-4009-89
Taille
127.8 mm × 85.5 mm × 30.6 mm
Matrice
highly cross-linked 6% agarose
Taille moy. des particules
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
Capacité
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Description générale
Application
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
Caractéristiques et avantages
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Protocoles
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to purify protein A-tagged proteins using Cytiva products.
How to isolate proteins from inclusion bodies using Cytiva products.
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