Accéder au contenu
Merck
Toutes les photos(1)

Documents

F8771

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Goat Anti-Mouse IgG (Fab specific)−Fluorescein isothiocyanate

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00162622
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

200

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:64

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Spécificité

Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG and rat serum proteins to ensure minimal cross reactivity.

Immunogène

Purified mouse IgG

Application

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:64. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:200. For immunofluorescence, a working dilution of 1:200 was used in various studies. To study DNA synthesis in mouse two-cell stage embryos by BrdU antibody, mouse IgG (Fab specific)-FITC antibody dilution of 1:50 was used.

Autres remarques

Antibody adsorbed with human IgG and rat serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background with human or rat samples

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Christine I Wooddell et al.
Current protocols in mouse biology, 1(4), 463-488 (2011-01-01)
Evans blue dye (EBD) can be used in live mice to study muscle pathology or injury, including exercise-induced muscle damage. EBD is excluded from intact cell membranes but leaks into cells, including muscle fibers, when the cell membrane is ruptured.
Sigrid Eckardt et al.
Reproduction (Cambridge, England), 129(5), 547-556 (2005-04-28)
Mammalian somatic cell cloning requires factors specific to the oocyte for reprogramming to succeed. This does not exclude that reprogramming continues during the zygote and cleavage stages. The capacity or role of zygotic and cleavage stages to reprogram somatic cell
P L Gabbott et al.
The Journal of comparative neurology, 350(2), 281-301 (1994-12-08)
The rationale for this study was to provide a comprehensive light microscopical description of the morphology of diaphorase-reactive neurons and neuropil elements in the dorsal lateral geniculate nucleus (dLGN) of the rat. An additional objective was to quantitatively assess whether
German Kamalov et al.
Journal of cardiovascular pharmacology, 62(6), 497-506 (2013-10-03)
Cardinal pathological features of hypertensive heart disease (HHD) include not only hypertrophied cardiomyocytes and foci of scattered microscopic scarring, a footprint of prior necrosis, but also small myocytes ensnared by fibrillar collagen where disuse atrophy with protein degradation would be
Elzbieta Pamula et al.
Journal of biomedical materials research. Part A, 89(2), 432-443 (2008-04-24)
Degradable three-dimensional porous scaffolds applicable as cell carriers for bone tissue engineering were developed by an innovative solvent casting/particulate leaching technique from poly(L-lactide-co-glycolide) (PLG). Three types of PLG scaffolds were prepared, and these had the same high porosity (83%) but
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique