The DMSO is stable at room or refrigerated temperatures. Typically, cell freezing media containing serum and DMSO, are stable for at least several days when thawed and refrigerated. Other constituents of freezing medium, primarily serum, may contribute to the appearance of particulates. For detailed troubleshooting, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further.
D2438
Diméthylsulfoxyde
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Dimethyl sulfoxide
Synonyme(s) :
DMSO, Sulfoxyde de méthyle
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A propos de cet article
Formule linéaire :
(CH3)2SO
Numéro CAS:
Poids moléculaire :
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Grade:
BioPerformance Certified
Technique(s):
cell culture | hybridoma: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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BioPerformance Certified
Quality Segment
agency
USP XXIV, meets EP testing specifications, meets USP testing specifications
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
sterility
sterile-filtered
form
liquid
autoignition temp.
573 °F
expl. lim.
42 %, 63 °F
technique(s)
cell culture | hybridoma: suitable, cryopreservation: suitable
impurities
≤0.1% water
color
colorless
refractive index
n
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
Le diméthylsulfoxyde (DMSO) est un solvant aprotique. Les molécules thérapeutiques et toxiques qui ne sont pas solubles dans l'eau sont souvent solubles dans le DMSO. L'association de l'azote liquide et d'un agent cryoprotecteur tel que le DMSO est couramment utilisée pour la conservation des cellules. Le DMSO permet de préparer des solutions mères de diverses molécules et sert également de témoin pour ces molécules. Il est utilisé dans les tests de migration cellulaire pour la dissolution des cellules post-migration. Le DMSO confirmé sans BPC par analyse répond également aux exigences des applications de biotechnologies et de génie tissulaire.
Application
Features and Benefits
Produit stérilisé par filtration et prêt à l'emploi, commodément conditionné dans des flacons ambrés à septum et bouchon à vis. Conforme aux exigences d'analyse de l'USP et de la Ph. Eur.
Disclaimer
Refroidit facilement et revient doucement à la température de la pièce. Les produits solides peuvent être reliquéfiés par chauffage à la température ambiante sans abîmer le produit.
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Cet article | |||
|---|---|---|---|
| description sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma | description BioUltra, Molecular Biology, ≥99.5% (GC) | description Pharmaceutical Secondary Standard; Certified Reference Material | description puriss. p.a., ACS reagent, ≥99.9% (GC) |
| grade BioPerformance Certified | grade Molecular Biology | grade certified reference material, pharmaceutical secondary standard | grade ACS reagent, puriss. p.a. |
| Quality Level 400 | Quality Level 200 | Quality Level 300 | Quality Level - |
| technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) GC/GC: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) GC/GC: suitable |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility - | solubility - |
| form liquid | form liquid | form - | form liquid |
| mp 16-19 °C (lit.) | mp 16-19 °C (lit.) | mp - | mp 16-19 °C (lit.), 18-20 °C |
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Classe de stockage
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Is it okay to mix it with freezing media that is kept refrigerated? Additionally, when I mixed DMSO with freezing media that was kept refrigerated, a white floating substance appeared. Do you know what might have caused this?
1 answer-
Helpful?
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For D2438-5X10ML, do they come in glass ampoule or bottle with lid?
1 answer-
Yes, both package sizes have a septum cap closure as shown in the image below:
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Hi, what is the difference between DMSO product# D2438 and D2650? What is Hybri-Max vs. BioPerformance certified and meets USP testing specification?
1 answer-
Both D2438 and D2650 are suitable for cell culture. The D2650 is part of the Hybri-Max™ product line. Hybri-Max products have been tested for use in hybridoma creation and culture. The D2438 is part of the BioPerformance Certified group of products. These products are tested and optimized for use in biological applications. For this product and a number of others, this includes testing to meet USP and EP guidelines. Please see the links below to review a sample Certificate of Analysis for each item.
D2650
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation
D2438
https://www.sigmaaldrich.com/product/sigma/d2650#product-documentationHelpful?
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Purity grade of the product ?
1 answer-
The purity of this product is listed on the lot specific Certificate of Analysis. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a Certificate:
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentationHelpful?
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El DMSO es sensible a la luz, hay que tomar alguna precaución al respecto?
1 answer-
This product is packaged in amber glass and is protected from light. The 10 mL size is packaged in a sealed ampule. Once opened, any content that is not used should also be stored in amber glass. For long term storage, the material may also be stored under inert gas to preserve the shelf life. Note that this material has a tendency to absorb water. Containers should be tightly sealed.
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what are the storing conditions?
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This product is stored at room temperature. Store in a well-ventilated place. Keep cool. Please see the Material Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/d2438.
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What is the difference between products D2438 and D8418?
1 answer-
The D2438 is a BioPerformance Certified grade product suitable for cell culture, hybridoma creation, and cryopreservation. It is intended for research use only but does meet USP and EP testing requirements. The D8418 is a molecular biology-grade product suitable for DNA sequencing, PCR, and transfection. It has been tested for RNAse and DNAse. The products can be selected based on the application.
Please view the sample Certificate of Analysis for each product to see the testing specifications associated with that item:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF734068.pdf
https://www.sigmaaldrich.com/coa/SIGMA/D2438/RNBL8837Helpful?
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What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?
1 answer-
The industry standard for 'endotoxin free' is less than 1 EU/mL.
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What is the DMSO dissolved in?
1 answer-
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
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Where does DMSO come from?
1 answer-
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
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