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D1946

Sigma-Aldrich

Diaminofluorescein-FM diacetate

≥98% (HPLC)

Synonyme(s) :

4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy), DAF-FM DA

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About This Item

Formule empirique (notation de Hill):
C25H18F2N2O7
Numéro CAS:
254109-22-3
Poids moléculaire :
496.42
Numéro MDL:
MFCD16872050
Code UNSPSC :
12352200
ID de substance PubChem :
329798717
Nomenclature NACRES :
NA.77

Niveau de qualité

100

Pureté

≥98% (HPLC)

Forme

film

Conditions de stockage

desiccated

Couleur

colorless to yellow

Fluorescence

λex 500 nm; λem 515 nm in DMSO

Conditions d'expédition

wet ice

Température de stockage

−20°C

Chaîne SMILES 

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

Clé InChI

BEVHTVRRVVEMEF-UHFFFAOYSA-N

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Application

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Actions biochimiques/physiologiques

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Caractéristiques et avantages

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Attention

Hygroscopic

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Concentration-dependent osteogenic and angiogenic biological performances of calcium phosphate cement modified with copper ions
Zhang J, et al.
Materials Science and Engineering, C, 99, 1199-1212 (2019)
Markus Schlicht et al.
Frontiers in plant science, 4, 351-351 (2013-09-24)
Powdery mildews are a diverse group of pathogenic fungi that can infect a large number of plant species, including many economically important crops. However, basic and applied research on these devastating diseases has been hampered by the obligate biotrophic lifestyle
Longmei Zhai et al.
Plant physiology and biochemistry : PPB, 109, 515-524 (2016-11-12)
To cope with iron (Fe) deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiological responses. Recent studies have demonstrated that nitric oxide (NO) is involved in the Fe-deficiency response through hormonal signaling
Bing Fang Luo et al.
Journal of experimental botany, 63(8), 3127-3136 (2012-03-02)
Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered
Shaoting Du et al.
Journal of experimental botany, 67(3), 893-904 (2015-11-27)
CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO)
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