251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• Synonyme(s) : DAF-2, 4,5-Diaminofluorescein
• Formule empirique (notation de Hill): C₂₀H₁₄N₂O₅
• Numéro CAS: 205391-01-1
• Poids moléculaire : 362.34
• Numéro MDL: MFCD05865100
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: solution
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: pale yellow
• Fluorescence: λ_ex ~495 nm; λ_em ~515 nm
• Température de stockage: −20°C
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• Clé InChI: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

Description

Description générale

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Actions biochimiques/physiologiques

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Avertissement

Toxicity: Irritant (B)

Forme physique

A 5 mM (1 mg in 550 µl) solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Informations légales

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Point d'éclair (°C): 87 °C - closed cup - (Dimethylsulfoxide)