PORTHOS

93122323, Porcine thyroid, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: PORTHOS, 93122323
• Source biologique: Porcine thyroid
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Thyroglobulin, plasminogen activator (u-PA)
• Récepteurs: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Porcine thyroid epithelial

Description de la lignée cellulaire

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

Application

Regulation of thyroid cell metabolism

Milieu de culture

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 ⁴ cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Autres remarques

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