CCRF S-180 II

90011901, mouse unknown/unspecified, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: CCRF S-180 II, 90011901
• Source biologique: mouse unknown/unspecified
• Mode de croissance: Adherent
• Caryotype: 2n = 40
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse CFW sarcoma 180

Description de la lignée cellulaire

Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.

Application

Chemotherapy screening tests

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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