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C8058

Sigma-Aldrich

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

Synonyme(s) :

Chondroitin sulfate B lyase

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About This Item

Numéro CAS:
52227-83-5
Numéro MDL:
MFCD00166444
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bacterial (Flavobacterium heparinum)

Niveau de qualité

200

Conjugué

(Glucosaminoglycan)

Forme

lyophilized powder (with BSA as stabilizer)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Application

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.

Actions biochimiques/physiologiques

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.

Définition de l'unité

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Consulter la Bibliothèque de documents

Yvette M Coulson-Thomas et al.
Journal of neuroscience methods, 171(1), 19-29 (2008-04-18)
Injury to the CNS of vertebrates leads to the formation of a glial scar and production of inhibitory molecules, including chondroitin sulphate proteoglycans. Various studies suggest that the sugar component of the proteoglycan is responsible for the inhibitory role of
Y M Michelacci et al.
The Biochemical journal, 151(1), 121-129 (1975-10-01)
A chondroitinase that degrades only chondroitin sulphate B was isolated from Flavobacterium heparinum, and separated from a constitutive chondroitinase AC also present in extracts of F. heparinum. The enzyme acts only on chondroitin sulphate B, producing oligo- and tetra-saccharides, plus
Zhenqing Zhang et al.
Analytical biochemistry, 385(1), 57-64 (2008-11-11)
Liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. Next
Nicola Volpi et al.
Glycobiology, 19(4), 356-367 (2008-12-06)
Glycosaminoglycans from the body of marine clam Scapharca inaequivalvis were extracted at about 0.15- 0.18 mg/g of dry tissue, composed of dermatan sulfate (DS) (approx. 74%) and heparan sulfate (26%). After treatment with nitrous acid, DS was isolated for further
Kristen R Taylor et al.
The Journal of biological chemistry, 280(7), 5300-5306 (2004-11-26)
Glycosaminoglycans have been implicated in the binding and activation of a variety of growth factors, cytokines, and chemokines. In this way, glycosaminoglycans are thought to participate in events such as development and wound repair. In particular, heparin and heparan sulfate
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