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A7104

Sigma-Aldrich

Anti-AtMPK6 antibody produced in rabbit

affinity isolated antibody, liquid

Synonyme(s) :

Anti-Arabidopsis thaliana MPK6

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About This Item

Numéro MDL:
MFCD07370261
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Qualité

for molecular biology

Clone

polyclonal

Forme

liquid

Poids mol.

antigen 47 kDa

Espèces réactives

Arabidopsis thaliana

Technique(s)

western blot: 0.25-0.5 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Arabidopsis thaliana ... ATMPK6(818982)

Description générale

In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant MAPKs are classified into the plant extracellular-regulated kinase/ERK (PERK) subfamily.

Immunogène

synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK6 (amino acids 384-395). This sequence is specific to AtMPK6 (not found in the other 20 known AtMPK isoforms). It shares limited homology (40-50% identity) to the AtMPK3 and AtMPK4 isoforms, and is highly conserved in the Nicotiana tabacum ortholog SIPK.

Application

Anti-AtMPK6 antibody produced in rabbit has been used in immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Mitogen-activated protein kinase (MAPK) cascades are among the most conserved signal transduction pathways in eukaryotes. MAPKs regulate a variety of cellular activities ranging from gene expression, cell proliferation, motility, metabolism and apoptosis. In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4 and AtMPK6 and all three MAPKs have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold and touch.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis
Zhang Z, et al.
Molecular plant-microbe interactions : MPMI, 30(2), 150-160 (2017)
Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases
MAP Kinases in Plant Signal Transduction (2012)
T Vavrdová et al.
Plant methods, 15, 22-22 (2019-03-23)
In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a considerable z-range and circumvent its low temporal resolution capacity
Emerging MAP kinase pathways in plant stress signalling
Nakagami H, et al.
Trends in Plant Science, 10(7), 339-346 (2005)
Charlotte H Hurst et al.
Plant physiology, 177(2), 522-531 (2018-04-25)
Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals
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