For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.
A50120
DEAE–Sephadex®
Synonyme(s) :
Diethylaminoethyl Sephadex®
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powder
Quality Segment
technique(s)
LPLC: suitable, protein purification: suitable
matrix
cross-linked dextran
bead size
40-125 μm (dry)
pore size
~200,000 Da exclusion limit
pH
2—12
capacity
3-4 meq/g binding capacity
General description
A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02
A50120-500G′s updated product number is GE17-0180-02
Application
DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.
Legal Information
Sephadex is a registered trademark of Cytiva
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| description - | description CL-6B | description - | description - |
| matrix cross-linked dextran | matrix 6% cross-linked agarose | matrix cross-linked dextran | matrix - |
| pore size ~200,000 Da exclusion limit | pore size ~4,000,000 Da exclusion limit | pore size ~30,000 Da exclusion limit | pore size ~200,000 Da exclusion limit |
| capacity 3-4 meq/g binding capacity | capacity 130-170 μeq/mL binding capacity (gel volume)(gel volume) | capacity 3-4 meq/g ion exchange capacity | capacity 4.0-5.0 meq/g ion exchange capacity |
| technique(s) LPLC: suitable, protein purification: suitable | technique(s) LPLC: suitable, affinity chromatography: suitable | technique(s) activity assay: suitable | technique(s) activity assay: suitable |
| form powder | form suspension | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?
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What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?
1 answer-
Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.
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How can Product A50120, DEAE-Sephadex® resin be regenerated?
1 answer-
Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.
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How can Product A50120, DEAE-Sephadex® resin, be stored?
1 answer-
Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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