A25120

DEAE-Sephadex^® A-25 chloride form

• Synonyme(s) : DEAE Sephadex^®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex^®
• Numéro CAS: 12609-80-2
• UNSPSC Code: 47101511
• NACRES: NA.56
• MDL number: MFCD00132778

Propriétés

• form: powder
• Quality Segment: 200
• technique(s): activity assay: suitable
• matrix: cross-linked dextran
• matrix active group: functional group
• bead size: 40-125 μm (dry)
• pore size: ~30,000 Da exclusion limit
• operating pH: 2-9
• capacity: 3-4 meq/g ion exchange capacity
• compatibility: mode of use mixed weak and strong anion exchange chromatography

Description

General description

The Sephadex^® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Application

DEAE-Sephadex^® A-25 has been used:

• to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
• in the estimation of GLS content in Chinese kale sprouts
• production and purification of endozepine from astrocytes
• to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Features and Benefits

• Weak anion exchanger
• Suitable for batch techniques
• High binding capacities
• Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Legal Information

DEAE Sephadex is a registered trademark of Cytiva

Sephadex is a registered trademark of Cytiva

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)