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A2055

Sigma-Aldrich

Tubes ACCUSPIN™, stériles, d′une capacité de 50 ml

radiation sterilized tube fitted with a high density polyethylene barrier

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Stérilité

sterile; irradiated

Forme

tubes

Application(s)

hematology
histology

Température de stockage

15-25°C

Catégories apparentées

Description générale

Les tubes ACCUSPIN™ sont des tubes en polypropylène stérilisés par rayonnement comportant une barrière en polyéthylène haute densité. Chaque tube peut contenir 15 ml de gradient de densité.

Application

Les tubes stériles ACCUSPIN™ d'une capacité de 50 ml sont prévus pour être utilisés avec le milieu de séparation à gradient de densité Histopaque™–1077 :


  • dans l'isolement de lymphocytes et autres cellules mononucléées.
  • dans l'isolement de cellules progénitrices endothéliales issues de sang de cordon ombilical[1].
  • dans l'isolement d'une couche de cellules mononucléées à partir d'échantillons de moelle osseuse.
  • Produit pouvant être utilisé pour la purification d'aspirats de moelle osseuse humaine dans le but d'étudier la valeur thérapeutique d'inhibiteurs de BET vis-à-vis de la leucémie myéloïde aiguë.

Principe

Après addition de milieu Histopaque-1077 au tube ACCUSPIN, une brève centrifugation permet de placer le milieu Histopaque-1077 sous le fritté. L'échantillon de sang peut être ajouté dans la chambre supérieure du tube sans risque qu'il soit mélangé avec le milieu Histopaque-1077 qui se trouve dans la chambre inférieure sous le fritté. Après une nouvelle centrifugation, le sang total descend à travers le fritté pour venir en contact avec le milieu Histopaque-1077. Les éléments plus denses déplacent un volume d'Histopaque-1077 au-dessus du fritté, ce qui permet de séparer clairement les constituants du sang. Les érythrocytes s'agrègent et les granulocytes deviennent légèrement hypertoniques, ce qui accroît leur vitesse de sédimentation et entraîne la formation d'un culot au fond du tube ACCUSPIN. Les lymphocytes et autres cellules mononucléées, comme les monocytes, restent à l'interface plasma/Histopaque-1077. Cette bande dense de cellules mononucléées peut être recueillie en versant le contenu de la chambre supérieure ou à l'aide d'une pipette. On évite ainsi toute contamination par les érythrocytes grâce à la barrière entre les chambres.

Quantité

10 tubes par pack.

Informations légales

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000411992
0000411992
0000367710
0000357493
0000293741

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Kara S Cox et al.
mAbs, 8(1), 129-140 (2015-10-23)
Monitoring antigen-specific memory B cells and the antibodies they encode is important for understanding the specificity, breadth and duration of immune response to an infection or vaccination. The antibodies isolated could further help design vaccine antigens for raising relevant protective
S A Mireku et al.
Scientific reports, 7(1), 14296-14296 (2017-11-01)
Bacterial ABC importers catalyze the uptake of essential nutrients including transition metals and metal-containing co-factors. Recently, an IgG antibody targeting the external binding protein of the Staphylococcus aureus Mn(II) ABC importer was reported to inhibit transport activity and reduce bacterial
Aimin Tang et al.
Nature communications, 10(1), 4153-4153 (2019-09-14)
Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months of age worldwide; therefore, the prevention of RSV infection in all infants represents a significant unmet medical need. Here we report the isolation
Juan M Melero-Martin et al.
Methods in enzymology, 445, 303-329 (2008-11-22)
Rapid and complete vascularization of ischemic tissues and thick engineered tissues is likely to require vasculogenesis. Therefore, the search for clinically relevant sources of vasculogenic cells and the subsequent development of experimental models of vasculogenesis is of utmost importance. Here
Samuel J S Rubin et al.
Nature communications, 10(1), 2686-2686 (2019-06-21)
Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalence and healthcare costs. Diagnosis
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Questions

1–5 of 5 Questions  

  1. How should a 4-20% sucrose gradient be used to reduce platelet contamination, as recommended in the user manual?

    1 answer

    1. Sucrose exerts an osmotic pressure of 300 mOsm at a concentration of about 0.25M, which is iso-osmotic with the contents of most mammalian cells. This concentration is approximately 81.25 grams/L or 8.125%, as per protocols used to remove platelets. Recommended procedures for removing platelets:
      Perform Procedure 1077 or 1077/1119.
      Collect the cell band and layer it over a second gradient.
      A. Bottom layer = 1077-1
      B. Top layer = 8.125% sucrose solution (prepared in deionized water).
      C. Layer the cell suspension on top of the gradient formed in steps 1-2.
      D. Use a 300 g spin for 10-15 minutes.
      E. Depending upon the original volume of the cell suspension, times and speed may have to be adjusted to maximize cell recovery.
      F. Use separate tubes for mononuclear and polymorphonuclear cells.

      Helpful?

  2. Is Accuspin (A2055) suitable for use with canine blood?

    1 answer

    1. The suggestion is to make a decision based on individual circumstances.If currently using a plain centrifuge tube for collecting PBMCs from canine blood using a single density gradient, the Accuspin Tubes can be used for the same collection, provided that the G force does not exceed 1000G and a single density gradient is used.

      Helpful?

  3. Does the Accuspin Tubes Product #A2055, which are prefilled with a frit, also contain EDTA or another anticoagulant?

    1 answer

    1. None of the Accuspin spin tubes (filled or unfilled) contain any anticoagulant. It is expected that when blood is drawn, it is typically drawn into a vacuum tube that already contains an anticoagulant or it is necessary to add anticoagulant immediately after the blood draw is completed. The most common anticoagulants used are EDTA and Heparin.

      Helpful?

  4. Are Accuspin tubes compatible with blood that has been anticoagulated with ACD-A?

    1 answer

    1. Certainly, Accuspin tubes are suitable for use with any blood containing an anticoagulant, with fresher blood being preferable. Regardless of the anticoagulant used, the anticoagulant is essentially washed away during the steps to remove the density gradient from the collected white blood cells. With ACD-A, the cell band is wider at the interface and the cells are more dispersed over a larger area, possibly due to the dextrose/glucose in the formulation. When using ACD-A, a somewhat unusual cell band is produced after the tubes are removed from the centrifuge. In comparison, cell bands with heparin, EDTA, sodium citrate, and other typical anticoagulants typically provide a rather narrow cell band when viewed from the side of the Accuspin tube.

      Helpful?

  5. Can I remove the Frit after collecting the mononuclear cells to recover the granulocytes?

    1 answer

    1. When using the Accuspin System to collect Peripheral Blood Mononuclear Cells (PBMCs), there is no expectation that the neutrophils can be recovered. Although it is possible to dislodge the frit, some granulocytes will be suspended in the lower layers of the density gradient. The remaining granulocytes will appear as a gray layer on top of the packed red blood cells.

      In theory, the end-user may be able to collect this buffy coat that sits atop the packed red blood cell layer. It is recommended to either perform a lysis step to lyse the red blood cells, or run the cells back over another density gradient - such as Histopaque 11191 that has a density of 1.119 g/ml.

      Instead of performing this 2-step procedure that has not been validated, the more logical option would be to simply run the whole peripheral blood over a 2-step discontinuous gradient. Currently, Histopaque 10771 and Histopaque 11191, are offered. The Histopaque 11191 instructions for use are available in the DOCUMENTATION section in the 'More Documents' tab of the product detail page:
      https://www.sigmaaldrich.com/product/sigma/a2055#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/193/872/a2055pis.pdf

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