75633
Osmium tetroxide solution
suitable for electron microscopy, 2% in H2O
Synonyme(s) :
Osmium(IV) oxide
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A propos de cet article
Formule linéaire :
OsO4
Numéro CAS:
Poids moléculaire :
254.23
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Form:
solution
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solution
Quality Segment
reaction suitability
reagent type: oxidant
concentration
2% in H2O
technique(s)
electron microscopy: suitable
color
colorless to yellow
density
1.0 g/mL at 20 °C
application(s)
hematology
histology
storage temp.
2-8°C
SMILES string
O=[Os](=O)(=O)=O
InChI
1S/4O.Os
InChI key
VUVGYHUDAICLFK-UHFFFAOYSA-N
General description
Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.
Application
Osmium tetroxide solution has been used as a fixative in electron microscopy.
Biochem/physiol Actions
Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.
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Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Classe de stockage
6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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