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51290

Sigma-Aldrich

Hemoglobin from bovine blood

lyophilized, 2×cryst., ≥90% (SDS-PAGE), powder

Synonyme(s) :

Bovine hemoglobin, Hb, Methemoglobin

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

bovine blood

Niveau de qualité

Essai

≥90% (SDS-PAGE)

Forme

powder

Qualité

2×cryst.
lyophilized

Poids mol.

Mr ~64500

Technique(s)

UV/Vis spectroscopy: suitable

Impuretés

salt, none detected
~0.3% Fe

Perte

≤8% loss on drying

Numéro d'accès UniProt

Température de stockage

2-8°C

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Description générale

Hemoglobin (Hb) is the protein responsible for storage and transport of oxygen and other gaseous ligands in red blood cells (RBC). Hb is also the pioneer material for synthesis of Hb-based oxygen carriers (HBOCs) that can be used as substitutes for RBC. [1]

Application

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Actions biochimiques/physiologiques

Oxygen transporter, NO scavenger
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Attention

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Autres remarques

Review: Oxygen carrier proteins[2]
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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Paul W Buehler et al.
Biomaterials, 31(13), 3723-3735 (2010-02-13)
Hemoglobin-based oxygen carriers (HBOC) are currently being developed as red blood cell (RBC) substitutes for use in transfusion medicine. Despite significant commercial development, late stage clinical results of polymerized hemoglobin (PolyHb) solutions hamper development. We synthesized two types of PolyHbs
M. Brunori et al.
Topics in Molecular and Structural Biology, 7, 263-263 (1985)
Kaori Yamazaki et al.
Clinical physiology and functional imaging, 33(2), 109-116 (2013-02-07)
Time-resolved spectroscopy (TRS-20) measures tissue oxygen saturation (%) by evaluating the absolute concentrations of oxygenated, deoxygenated and total haemoglobin based on measurement of the transit time of individual photons through a tissue of interest. We measured tissue oxygen saturation in
J L Schei et al.
Neuroscience, 227, 191-200 (2012-10-09)
Coupling between neural activity and hemodynamic responses is important in understanding brain function, interpreting brain-imaging signals, and assessing pathological conditions. Tissue state is a major factor in neurovascular coupling and may alter the relationship between neural and hemodynamic activity. However
Dafna Ben Bashat et al.
PloS one, 7(11), e49416-e49416 (2012-12-05)
Blood oxygenation level dependence (BOLD) imaging under either hypercapnia or hyperoxia has been used to study neuronal activation and for assessment of various brain pathologies. We evaluated the benefit of a combined protocol of BOLD imaging during both hyperoxic and

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