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SE100

Sigma-Aldrich

Silver Enhancer Kit

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About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Forme

liquid

Niveau de qualité

Utilisation

sufficient for ≥100 slides staining and fixing

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Caractéristiques et avantages

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Conditionnement

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principe

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Composants de kit seuls

Réf. du produit
Description

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • S5020Silver enhancer solution A 100 mLFDS

  • S5145Silver enhancer solution B 100 mLFDS

Pictogrammes

Health hazardEnvironment

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Using Immuno-Scanning Electron Microscopy
for the Observation of Focal Adhesion-substratum
interactions at the Nano- and Microscale in S-Phase Cells
Manus J.P. Biggs
Cell Culture Labfax (2011)
Kathleen Grennan et al.
Talanta, 68(5), 1591-1600 (2008-10-31)
With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation
A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Hadiyah N
International journal of nanomedicine (2014)
Tsung-Ting Tsai et al.
Scientific reports, 7(1), 3155-3155 (2017-06-11)
In this study, we investigated the antibacterial activity of silver-coated gold nanoparticles (Au-Ag NPs) immobilized on cellulose paper. Ag NPs are known to have strong antibacterial properties, while Au NPs are biocompatible and relatively simple to prepare. We made the
Yuan Chen et al.
Oncotarget, 9(41), 26556-26571 (2018-06-15)
Tumor hypoxia is a well-recognized driver of resistance to traditional cancer therapies such as chemotherapy and radiation therapy. We describe development of a new nanoconstruct composed of gold nanorods (GNRs) conjugated to carbonic anhydrase IX (CAIX) antibody that specifically binds

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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