Direct Red 80 is not performance tested for use in a Picro Sirius Red staining method. Tissues fixed or stained with Picric Acid may require long rinse or wash steps to remove excess picric acid, and using slightly acidified water has been seen in protocols to remove the excess picric acid from tissue sections.
365548
Direct Red 80
Dye content 25 %
Synonyme(s) :
Sirius Red
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About This Item
Formule empirique (notation de Hill):
C45H26N10Na6O21S6
Numéro CAS:
Poids moléculaire :
1373.07
Numéro C.I. (Colour Index):
35780
Beilstein:
8185575
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.47
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
powder
Niveau de qualité
Composition
Dye content, 25%
Technique(s)
titration: suitable
Couleur
dark brown
λmax
528 nm
ε (coefficient d'extinction)
≥12000 at 277-283 nm in H2O
≥15000 at 223-235 nm in H2O
≥32000 at 524-530 nm in H2O
Application(s)
diagnostic assay manufacturing
hematology
histology
Température de stockage
room temp
Chaîne SMILES
[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O
InChI
1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;
Clé InChI
YIQKLZYTHXTDDT-IJPLDVMMSA-H
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Catégories apparentées
Description générale
Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.
Application
Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.Other applications include -
- detection of amyloid
- Sirius red-Van Gieson methods for quantitation of collagen using image analysis methods
- counterstaining applications substituting eosin in alum hematoxylins
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Using Direct Red 80 with Picric acid P6744 for Picro Sirius Red staining, and the staining resultss are coming out very yellow. Any advice on this issue?
1 answer-
Helpful?
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Is Direct Red 80 comparable to Dextran Red for back-filling injections in live zebrafish spinal cord?
1 answer-
Direct Red 80 is more commonly referred to as Sirius Red and is a stain for collagen. It is a vital stain used for various types of biological staining. Dextran Red is a fluorescent dye for which you would require a fluorescent microscope to view the staining. The different molecular weights of dextran are used to determine whether the dye crosses certain barriers.
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