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Merck
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Principaux documents

10981

Supelco

Polyvinyl alcohol mounting medium with DABCO®, antifading

pH 8.7

Synonyme(s) :

PVA-DABCO®

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About This Item

Code UNSPSC :
12000000
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Indice de réfraction

n20/D 1.377

Niveau de qualité

100

pH

8.7

Densité

1.08 g/mL at 20 °C

Adéquation

in accordance for application

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Forme physique

Aqueous solution containing tris(hydroxymethyl)aminomethane, tris(hydroxymethyl)aminomethane hydrochloride, polyvinyl alcohol 22000, glycerol, and 1,4-diazabicyclo[2.2.2]octane

Informations légales

DABCO is a registered trademark of Air Products & Chemicals, Inc.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H318

Conseils de prudence

P280 - P305 + P351 + P338

Classification des risques

Eye Dam. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

212.0 °F - closed cup

Point d'éclair (°C)

100 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCM7403
BCCK8672
BCCL5464
BCCK0370
BCCH5688

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Anti-DCX (AB1) antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

AV41333

Anti-DCX (AB1) antibody produced in rabbit

1,4-Diazabicyclo[2.2.2]octane for synthesis

Sigma-Aldrich

8.03456

1,4-Diazabicyclo[2.2.2]octane

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Questions

1–10 of 10 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What are the instructions for using Polyvinyl Alcohol with DABCO 10971?

    1 answer

    1. There is no available information regarding using Polyvinyl Alcohol with DABCO 10971. Basic information is available that Polyvinyl Alcohol solution is ready to use as it comes from the bottle and should be stored at a temperature of 2-8°C and is important to bring the aqueous mounting media to room temperature before use.

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  4. After mounting the slides should be stored at room temperature or is better to have them at 2-8° or frozen at -20°C?

    1 answer

    1. After applying the mounting medium, slides should be stored at 4°C.

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  5. How does the use of aqueous Polyvinyl alcohol mounting medium with DABCO®, Product 10981, with an anti-fade agent differ from the use of a standard aqueous mounting media?

    1 answer

    1. The anti-fade agent used in product 10981 will result in an initial drop in the intensity of the fluorescence.  Once the drop in fluorescence has occurred, the remaining fluorescence is not prone to rapid photo bleaching.

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  6. Polyvinyl alcohol mounting medium with DABCO®, Product 10981, has gelled during refrigeration.  Is the product still usable?

    1 answer

    1. Yes, the product is still usable after gelling.  The product will however need to be reheated to around 50 degrees Celsius or higher.  It may take some time for the product to turn back into a liquid.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. Is Polyvinyl alcohol mounting medium with DABCO®, Product 10981, a permanent mounting media?

    1 answer

    1. No. This is considered an aqueous mounting media.  Although an antifade agent is included in the formulation, the fluorescence will eventually fade.

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  9. Can clear finger nail polish be used to replace Polyvinyl alcohol mounting medium with DABCO®, Product 10981, to seal the edges of the coverslips?

    1 answer

    1. Anecdotal evidence suggests that permanent mounting medias do a better job of stopping evaporation of the aqueous mounting media. Clear finger nail polish has been used by some labs for sealing the edges of the coverslip.  We are unaware of any formal studies which have been performed that would document which product gives the better performance.

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  10. Is it necessary to seal the slides after coverslipping?

    1 answer

    1. No. The slides do not require sealing after coverslipping. However sealing the edges of the coverslip with a permanent mounting media such as DPX will prevent the gradual formation of air bubbles as the aqueous mounting media gradually dries.

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