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Key Documents

WBULS0100

Millipore

Immobilon ECL Ultra Western HRP Substrate

Western blotting, HRP

Synonyme(s) :

HRP substrate

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About This Item

Code UNSPSC :
41116010
eCl@ss :
42029053
Nomenclature NACRES :
NB.22

Niveau de qualité

Technique(s)

western blot: suitable

Méthode de détection

chemiluminescent

Conditions d'expédition

ambient

Description générale

Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.

Application

Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.

Sufficient for 1000 cm²

Composants

Solution A- 50 mL

Solution B- 50 mL

Stockage et stabilité

Store at 2–8 °C

Informations légales

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°C)

Not applicable


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Articles

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocoles

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

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