71285-M
BCA Protein Assay Kit
The BCA protein assay is a simple and reliable protein quantification method.
Synonyme(s) :
BCA Protein Quantification, Bicinchoninic Acid Assay, Protein Assay
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About This Item
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.84
Produits recommandés
Fabricant/nom de marque
Novagen®
Conditions de stockage
OK to freeze
Température de stockage
10-30°C
Description générale
The BCA protein assay is a simple and reliable protein quantification method.
The BCA protein assay is based on a biuretreaction, which is the reduction of Cu2+ toCu+ by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. Bicinchoninic acidis a chromogenic reagent that chelates thereduced copper, producing a purple complexwith strong absorbance at 562 nm (Smith1985, Wiechelman 1988). This assay can beused to quantify protein concentration with awide variety of samples and can be performedin minutes.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
Composants
•500 mlBCA Solution
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
Avertissement
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Autres remarques
Smith, P.K., et al. 1985. Anal. Biochem.150, 76.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
Informations légales
Use of the BCA protein assay is permitted for research purposes only.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
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