SCC117

Histone H2B-GFP expressing HeLa Cell Line

Human

• Synonyme(s) : Histone H2B
• Code UNSPSC : 41106514
• eCl@ss : 32011203
• Nomenclature NACRES : NA.81

Propriétés

• product name: Histone H2B-GFP expressing HeLa Cell Line, The Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics.
• Source biologique: human
• Niveau de qualité: 100
• Technique(s): cell based assay: suitable; cell culture | mammalian: suitable
• Conditions d'expédition: ambient

Description

Description générale

Chromatin, the higher order structure of DNA, proteins and RNA, constitutes the majority of the nucleus of eukaryotic cells. Changes in chromatin structure are the essence of many essential nuclear processes, including transcription, meiosis, mitosis, and apoptosis. The nucleosome represents the primary building block of chromatin and it comprises an octomer of four core histone proteins (H2A, H2B, H3 and H4). HeLa cells expressing green fluorescent protein fused histone H2B (H2B-GFP) have been widely used to visualize the dynamics of chromosomal architecture in living cells during various processes. In addition to studying normal mitosis, histone H2B-GFP has been utilized for imaging the distinctive clustering behavior of double minute chromosomes (DMs) in cancer cells during mitosis, which contributes to their asymmetric distribution to daughter cells. Monitoring histone H2B-GFP also permits continuous analysis of chromosomal degradation during apoptosis.

Description de la lignée cellulaire

Cancer Cells

Application

Research Category
Apoptosis & Cancer

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Chromatin Biology

Histones

This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Qualité

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Stockage et stabilité

Histone H2B-GFP expressing HeLa Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Pictogrammes: GHS06
• Mention d'avertissement: Danger
• Mentions de danger: H301
• Conseils de prudence: P264 - P270 - P301 + P310 - P405 - P501
• Classification des risques: Acute Tox. 3 Oral
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2