Accéder au contenu
Merck
Toutes les photos(1)

Documents

293T cell line human

12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

Synonyme(s) :

293T cell line human, 293 T, 293tsA1609neo, 293tsA1609neo Cells, HEK-293-T Cells, HEK-293T Cells, HEK293T Cells, Human Embryonic Kidney 293T Cells

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41106514

product name

293T cell line human, 12022001

Source biologique

human kidney (fetal)

Mode de croissance

adherent

Morphologie

small slightly rounded cells growing in a dispersed manner

Produits

SV40 T antigen

Conditions d'expédition

dry ice

Température de stockage

−196°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line. It expresses the large T antigen of simian virus 40 (SV40).

Description de la lignée cellulaire

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Application

293T cell line human has been used:
  • for lentiviral particle production
  • in the expression of eukaryotic proteins
  • for small interfering RNA (siRNA) transfection
  • for human immunodeficiency virus (HIV) transfection for pseudoviruses production
  • as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression

Actions biochimiques/physiologiques

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.

Profil d'ADN

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Milieu de culture

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique